Rabbit Anti-SNX18 (Sorting Nexin-18, SH3 and PX Domain-containing Protein 3B, Sorting Nexin-associated Golgi Protein 1, SNX18, SH3PXD3B, SNAG1)
Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa449-628 of human SNX18.
Form
Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SNX18. Species Crossreactivity: mouse
