Mouse Anti-CCNF (FBX1, FBXO1)
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40aa motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq]
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MGSGGVVHCRCAKCFCYPTKRRIRRRPRNLTILSLPE DVLFHILKWLSVEDILAVRAVHSQLKDLVDNHASVWAC ASFQELWPSPGNLKLFERAAEKGNFEAAVKLGIAYLYN EGLSVSDEARAEVNGLKASRFFSLAERLNVGAAPFIWL FIRPPWSVSGSCCKAVVHESLRAECQLQRTHKASILH CLGRVLSLFEDEEKQQQAHDLFEEAAHQGCLTSSYLL WESDRRTDVSDPGRCLHSFRKLRDYAAKGCWEAQLS LAKACANANQLGLEVRASSEIVCQLFQASQAVSKQQV FSVQKGLNDTMRYILIDWLVEVATMKDFTSLCLHLTVE CVDRYLRRRLVPRYRLQLLGIACMVICTRFISKEILTIRE AVWLTDNTYKYEDLVRMMGEIVSALEGKIRVPTVVDY KEVLLTLVPVELRTQHLCSFLCELSLLHTSLSAYAPARL AAAALLLARLTHGQTQPWTTQLWDLTGFSYEDLIPCVL SLHKKCFHDDAPKDYRQVSLTAVKQRFEDKRYGEISQ EEVLSYSQLCAALGVTQDSPDPPTFLSTGEIHAFLSSP SGRRTKRKRENSLQEDRGSFVTTPTAELSSQEETLLG SFLDWSLDCCSGYEGDQESEGEKEGDVTAPSGILDVT VVYLNPEQHCCQESSDEEACPEDKGPQDPQALALDT QIPATPGPKPLVRTSREPGKDVTTSGYSSVSTASPTSS VDGGLGALPQPTSVLSLDSDSHTQPCHHQARKSCLQ CRPPSPPESSVPQQQVKRINLCIHSEEEDMNLGLVRL
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Protein corresponding to aa1-786 from full length human CCNF.
Form
Supplied as a liquid in PBS, pH 7.4.
Specificity
Recognizes human CCNF.