Mouse Anti-VMD2 (ARB, BEST, BMD, TU15B, VMD2)
This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYE NLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLG NVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEK LSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSL LNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFF LTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVG WLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVD EMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRVS FMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGL QSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAK QNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQ TPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSS GAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDM PEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSV LHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTL DLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFL GPGEGR
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Protein corresponding to aa1-604 from full length human VMD2
Form
Supplied as a liquid. No preservative added.
Specificity
Recognizes human VMD2.
