Technical Data

474370
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
IHC411
Grade
Affinity Purified
Applications
E IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PDL-1 (Programmed Cell Death 1 Ligand-1, CD274, Programmed Death Ligand 1, PD-L1, B7-H1)

Programmed Death-Ligand 1 (PD-L1), also known as CD274 or B7 Homolog 1 (B7-H1), is a transmembrane protein involved in suppressing the immune system and rendering tumor cells resistant to CD8+ T cell-mediated lysis through binding of the Programmed Death-1 (PD-1) receptor. Overexpression of PD-L1 may allow cancer cells to evade the actions of the host immune system. In renal cell carcinoma, upregulation of PD-L1 has been linked to increased tumor aggressiveness and risk of death, and, in ovarian cancer, higher expression of this protein has lead to significantly poorer prognosis. PD-L1 has also been linked to systemic lupus erythematosus and cutaneous melanoma. When considered in adjunct with CD8+ tumor-infiltrating lymphocyte density, expression levels of PD-L1 may be a useful predictor of multiple cancer types, including stage III non-small cell lung cancer, hormone receptor negative breast cancer, and sentinel lymph node melanoma.

Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:50-1:200 Immunohistochemistry (paraffin): 1:50-1:200 Optimal dilutions to be determined by the researcher.
Positive Control
Tonsil, Lung Adenocarcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human PD-L1.
Form
Supplied as a liquid in Tris, pH 7.3-7.7, 1% BSA, 0.09% sodium azide.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human PD-L1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ostrand-Rosenberg S, et al. J Immunol. 2014; 193:3835-41. |2. Tokito T, et al. Eur J Cancer. 2016; 55:7-14. |3. Park IH, et al. Clin Breast Cancer. 2016; 16:51-8. |4. Kakavand H, et al. Mod Pathol. 2015; 28:1535-44. |5. Xia B, et al. Immunotherapy. 2016; 8:279-98. |6. Patel SP, et al. Mol Cancer Ther. 2015; 14:847-56. |7. Singh BP, et al. Cancers (Basel). 2016; 8. |8. Chemnitz JM, et al. J Immunol. 2004; 173:945–54. |9. Thompson RH, et al. Proc Natl Acad Sci USA. 2004; 101:17174–9.|10. Hamanishi J, et al. Proc Natl Acad Sci USA. 2007; 104:3360–5.|11. Mozaffarian N, et al. Rheumatology, 2008; 47:1335–41.
USBio References
No references available
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