Technical Data

501627
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Gene ID
2162
Gene #
F13A1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-F13A1, NT
Coagulation factor XIII A chain, Coagulation factor XIIIa, 2.3.2.13, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain, F13A1, F13A

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

Applications
Suitable for use in Indirect Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Indirect Flow Cytometry: 1:25 Western Blot: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 170-204 of human F13A1.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human F13A1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ichinose A.,et al.Biochemistry 25:6900-6906(1986).|2. Grundmann U.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:8024-8028(1986).|3. Ichinose A.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:5829-5833(1988).|4. Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.|5. Mungall A.J.,et al.Nature 425:805-811(2003).
USBio References
No references available
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