Technical Data

502188
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Rt
Gene ID
84108
Gene #
PCGF6
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PCGF6, ID
Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134, PCGF6, MBLR, RNF134

Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within aa215-249 of the Central region of human PCGF6.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human PCGF6. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Akasaka T.,et al.Genes Cells 7:835-850(2002).|2. Ota T.,et al.Nat. Genet. 36:40-45(2004).|3. Deloukas P.,et al.Nature 429:375-381(2004).|4. Lee M.G.,et al.Cell 128:877-887(2007).|5. Maertens G.N.,et al.PLoS ONE 4:E6380-E6380(2009).
USBio References
No references available
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