Technical Data

502523
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
1141CT26.2.1
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_009330.1, NP_644671.1
Gene ID
6772
Gene #
STAT1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-STAT1
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine-and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified protein corresponding to human STAT1, fused to His-Tag.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human STAT1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kristensen I., et al. Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.|2. Schindler C., et al. Proc. Natl. Acad. Sci. U.S.A. 89:7836-7839(1992).|3. Ota T., et al. Nat. Genet. 36:40-45(2004).|4. Bechtel S., et al. BMC Genomics 8:399-399(2007).|5. Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
USBio References
No references available
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