Technical Data

502662
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
1340CT704.170.140
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Gene ID
8078
Gene #
USP5
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-USP5
Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5, USP5, ISOT

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human USP5.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human USP5. Species Crossreactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Falquet L.,et al.FEBS Lett. 376:233-237(1995).|2. Ansari-Lari M.A.,et al.Genome Res. 6:314-326(1996).|3. Ansari-Lari M.A.,et al.Genome Res. 7:268-280(1997).|4. Tashayev V.L.,et al.Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.|5. Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net