Rat Anti-F4/80
EGF-like module; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 receptor; Em Gpf4; TM7; Ly-71; f480; f-480; f4 80; f 480; f-4/80; f4-80
Recombinant monoclonal antibody to F4/80. Manufactured using recombinant Platform with variable regions (i.e. specificity) from the hybridoma Cl:A3-1 (recombinant version).
F4/80 is a 160kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Thioglycollate stimulated peritoneal macrophages of mouse origin.
Form
Supplied as a liquid in PBS, 0.02% Proclin 300.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse F4/80.
