Mouse Anti-Multidrug Resistance 3 (MDR3, ATP Binding Cassette Subfamily B Member 4, ABCB4)
Multidrug Resistance 3 (MDR3), also known as ATP Binding Cassette Subfamily B Member 4 (ABCB4), is a membrane-associated protein belonging to the superfamily of ATP-binding cassette transporters. MDR3 is an energy-dependent phospholipid efflux translocator that mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte, and also acts as a positive regulator of biliary lipid secretion. Defects in MDR3 are associated with progressive familial intrahepatic cholestasis type 3 and gallbladder disease type 1. Co-overexpression of MDR3 and MRP1 has been documented as correlating with blastemal subtype and high-risk prognosis of Wilms’ tumor patients.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:50-1:200 Immunohistochemistry (paraffin): 1:50-1:200 Optimal dilutions to be determined by the researcher.
Positive Control
Adrenal Cortex
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human Multidrug Resistance 3 (MDR3)
Form
Supplied as a liquid in Tris, pH 7.3-7.7, 1% BSA, 0.09% sodium azide.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Multidrug Resistance 3 (MDR3).
