Rabbit Anti-ADAM 17
A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), A disintegrin and metalloproteinase domain 17, ADA17_HUMAN, ADAM 17, ADAM metallopeptidase domain 17, ADAM17, ADAM17 protein, CD 156b, CD156b, CD156b antigen, CSVP, Disintegrin and metalloproteinase domain-containing protein 17, MGC71942, NISBD, NISBD1, Snake venom like protease, Snake venom-like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme, TNF-alpha convertase, TNF-alpha-converting enzyme, Tumor Necrosis Factor Alpha Converting Enzyme
This gene encodes a disintegrin and metalloprotease (ADAM) domain 17, which is a member of the ADAM protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. This gene contains 19 exons and alternative splicing generates 2 transcripts.
Applications
Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human ADAM 17.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human ADAM 17. Species Crossreactivity: Mouse and rat
