Rabbit Anti-AKAP13
A kinase (PRKA) anchor protein 13, A kinase anchor protein 13, A kinase anchoring protein, A-kinase anchor protein 13, AKAP 13, AKAP Lbc, AKAP-13, AKAP-Lbc, AKAP13, AKP13_HUMAN, ARHGEF13, Breast cancer nuclear receptor binding auxiliary protein, Breast cancer nuclear receptor-binding auxiliary protein, BRX, c lbc, FLJ11952, FLJ43341, Guanine nucleotide exchange factor Lbc, HA 3, HA3, Ht 31, Ht31, Human thyroid anchoring protein 31, Human thyroid-anchoring protein 31, LBC, LBC oncogene, Lymphoid blast crisis oncogene, Non oncogenic Rho GTPase specific GTP exchange factor, Non-oncogenic Rho GTPase-specific GTP exchange factor, p47, Protein kinase A anchoring protein 13, Protein kinase A-anchoring protein 13, PROTO LB, PROTO LBC
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to AKAP13.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total AKAP13. Species Crossreactivity: Human and mouse