Rabbit Anti-CD133
AC133, Antigen AC133, CD133, CORD12, Hematopoietic stem cell antigen, hProminin, MCDR2, MSTP061, OTTHUMP00000217744, OTTHUMP00000217745, OTTHUMP00000217746, PROM1, PROM1_HUMAN, Prominin I, Prominin like 1, Prominin like protein 1 precursor, Prominin mouse like 1, Prominin-1, Prominin-like protein 1, Prominin1, PROML1, RP41, STGD4
CD133, originally known as AC133. CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1). Currently the function of CD133 is unknown. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. CD133 is expressed in hematopoietic stem cells
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot:1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:200 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human CD133.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human CD133. Species Crossreactivity: Mouse and rat
