Rabbit Anti-EPHA5 (Ephrin Type-A Receptor 5, Tyrosine-protein Kinase Receptor EHK-1, Eph Homology Kinase-1, Receptor Protein-tyrosine Kinase HEK7, EPH-like Kinase 7, EK7, EHK1, HEK7)
Brain-specific kinase, bsk, CEK 7, Eck like sequence 1, EHK 1, EHK-1, EK7, Els 1, EPH homology kinase 1, EPH-like kinase 7, EPHA5, EPHA5_HUMAN, Ephrin type A receptor 5, Ephrin type-A receptor 5, HEK 7, hEK7, Receptor protein tyrosine kinase HEK 7, Rek 7, TYRO 4, Tyrosine protein kinase receptor EHK 1
EPH receptor A5 (ephrin type-A receptor 5) is a protein that in humans is encoded by the EPHA5 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human EPHA5.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human EPHA5. Species Crossreactivity: mouse and rat
