2BP1, A2 BP1, A2BP 1, A2BP, A2BP1, Ataxin 2 binding protein 1, Ataxin-2-binding protein 1, Ataxin2 binding protein 1, FOX 1, Fox-1 homolog A, fox-1-like RNA-binding protein 1, Hexaribonucleotide binding protein 1, Hexaribonucleotide-binding protein 1, HRNBP 1, HRNBP1, RBFOX1, RFOX1_HUMAN, RNA binding protein fox-1 homolog 1, RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1, RNA binding protein, fox-1 homolog (C. elegans) 1
RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human FOX1 at N-terminal. Species sequence homology: porcine (100%), bovine (100%), equine (100%), sheep (100%), canine (100%), chicken (92%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human FOX1. Species Crossreactivity: mouse and rat
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