Rabbit Anti-GPR31
12-(S)-HETE acid receptor, 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor, 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor, 12-(S)-HYDROXYEICOSATETRAENOIC ACID RECEPTOR, 12-HETER, bA517H2.2 (G protein coupled receptor 31), G protein coupled receptor 31, GPR31, HETER, HETER1, OTTHUMP00000017616, Probable G protein coupled receptor 31,
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human GPR31. Species sequence homology: porcine (%), bovine (%), equine (%), sheep (%), canine (%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human GPR31.