Rabbit Anti-KDM5A
Histone demethylase JARID1A, JARID1A, Jumonji/ARID domain containing protein 1A, Jumonji/ARID domain-containing protein 1A, Kdm5a, KDM5A_HUMAN, Lysine-specific demethylase 5A, RBBP-2, RBBP2, RBP2, Retinoblastoma binding protein 2, Retinoblastoma-binding protein 2
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human KDM5A at C-terminal. Species sequence homology: porcine (91%), equine (100%), canine (100%), chicken (100%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human KDM5A. Species Crossreactivity: mouse
