Rabbit Anti-Lck
IMD22, LCK, Lck p56, LCK proto-oncogene, Src family tyrosine kinase, LCK_HUMAN, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell specific protein tyrosine kinase, Lymphocyte cell-specific protein-tyrosine kinase, Lymphocyte specific protein tyrosine kinase, Membrane associated protein tyrosine kinase, Oncogene lck, P56 LCK, p56(LSTRA) protein tyrosine kinase, p56-LCK, p56lck, pp58 lck, pp58lck, Protein YT16, Proto oncogene tyrosine protein kinase LCK, Proto-oncogene Lck, Protooncogene tyrosine protein kinase LCK, T cell specific protein tyrosine kinase, T cell-specific protein-tyrosine kinase, T lymphocyte specific protein tyrosine kinase p56lck, Tyrosine-protein kinase Lck, YT 16, YT16
Tyrosine kinase that plays an essential role for the selection and maturation of developing t-cell in the thymus and in mature t-cell function
Applications
Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human Lck.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human Lck. Species Crossreactivity: mouse and rat
