Rabbit Anti-MTA1
Metastasis associated 1, Metastasis associated gene 1, Metastasis associated gene 1 protein, Metastasis associated protein, Metastasis associated protein MTA 1, Metastasis associated protein MTA1, Metastasis-associated protein MTA1, MTA 1, MTA1, MTA1_HUMAN
May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non- genomic responses of ER.
Applications
Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human MTA1. Species sequence homology: Porcine (91%), Bovine (91%), Equine (91%), Rabbit (91%), Canine (100%), Chicken (82%), Xenopus (80%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human MTA1. Species Crossreactivity: Mouse and rat
