Rabbit Anti-POLD3
DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, DNA polymerase delta, subunit 3, DNA polymerase subunit delta p66, DPOD3_HUMAN, KIAA0039, P66, P68, Pol delta C subunit (p66), Pold3, Polymerase (DNA directed), delta 3, Polymerase (DNA-directed), delta 3, accessory subunit, PPP1R128, Protein phosphatase 1, regulatory subunit 128
Applications
Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to POLD3. Species sequence homology: Porcine (82%), Bovine (91%), Equine (90%), Sheep (91%), Rabbit (91%), Canine (91%), Chicken (80%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total POLD3. Species Crossreactivity: Human, mouse and rat
