Rabbit Anti-PTK7
8430404F20Rik, CCK 4, CCK-4, Colon carcinoma kinase 4, Human colon carcinoma kinase 4 (CCK4) mRNA complete cds, Inactive tyrosine-protein kinase 7, mPTK7/CCK4, OTTHUMP00000217016, Protein tyrosine kinase PTK 7, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, PTK 7, PTK7, PTK7 protein tyrosine kinase 7, PTK7 protein tyrosine kinase, PTK7_HUMAN, Serum response factor, Srf, Tyrosine-protein kinase-like 7,
Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human PTK7. Species sequence homology: porcine (100%), bovine (100%), equine (100%), sheep (100%), rabbit (100%), canine (100%), chicken (83%), xenopus (83%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human PTK7. Species Crossreactivity: mouse
