Rabbit Anti-PTP1B
PTP1B, Non receptor tyrosine phosphatase 1, Protein phosphotyrosylphosphatase 1B, Protein tyrosine phosphatase 1B, Protein tyrosine phosphatase non receptor type 1, Protein tyrosine phosphatase placental, Protein-tyrosine phosphatase 1B, PTN1_HUMAN, PTP 1B, PTP-1B, PTPN 1, PTPN1, Tyrosine protein phosphatase non receptor type 1, Tyrosine-protein phosphatase non-receptor type 1
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human PTP1B.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human PTP1B. Species Crossreactivity: Mouse and rat
