Rabbit Anti-PYK2, phosphorylated (Tyr580)
CADTK, CAK-beta, CAKB, CAKbeta, Calcium regulated non receptor proline rich tyrosine kinase, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, E430023O05Rik, EC 2.7.10.2, FADK 2, FADK2, FAK2, FAK2_HUMAN, Focal adhesion kinase 2, MGC124628, PKB, Proline-rich tyrosine kinase 2, Protein kinase B, Protein Tyrosine Kinase 2 Beta, Protein-tyrosine kinase 2-beta, PTK, PTK2B, PTK2B protein tyrosine kinase 2 beta, PYK2, RAFTK, RAFTK2, Related adhesion focal tyrosine kinase
Pyk2 a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human PYK2 around the phosphorylation site of Tyrosine 580.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human PYK2 only when phosphorylated at Tyrosine 580. Species Crossreactivity: mouse and rat
