Rabbit Anti-RAB11FIP5
GAF 1, Gaf-1, GAF1, gamma SNAP associated factor 1, Gamma-SNAP-associated factor 1, KIAA0857, Phosphoprotein pp75, pp75, RAB11 family interacting protein 5 (class I), Rab11 family-interacting protein 5, Rab11 FIP5, Rab11 interacting protein, Rab11 interacting protein Rip11, Rab11-FIP5, Rab11-interacting protein Rip11, Rab11fip5, RFIP5_HUMAN, RIP11
Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight. ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human RAB11FIP5 at C-terminal. Species sequence homology: porcine (100%), bovine (100%), equine (100%), canine (100%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human RAB11FIP5. Species Crossreactivity: mouse, rat and monkey
