Rabbit Anti-TNNT3
AMCD2B, Beta TnTF, Beta-TnTF, DA2B, DKFZp779M2348, fast skeletal muscle, Fast skeletal muscle troponin T, FSSV, fTnT, TNNT 3, Tnnt3, TNNT3_HUMAN, TnTf, Troponin T, Troponin T fast skeletal muscle, Troponin T type 3 (skeletal fast), Troponin T type 3, Troponin T3 skeletal fast
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium- sensitivity to striated muscle actomyosin ATPase activity.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to TNNT3. Species sequence homology: Porcine (92%), Bovine (92%), Equine (92%), Canine (92%), Chicken (92%)
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total TNNT3. Species Crossreactivity: Human and mouse
