Rabbit Anti-Insulin R
INSR; Insulin receptor; IR; CD antigen CD220
INSR encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.
Applications
Suitable for use in Western Blot, Immunohistochemistry, and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin): 1:100-1:300 ELISA: 1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human Insulin R around the non-phosphorylation site of T1375.
Form
Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human Insulin R. Species Crossreactivity: mouse and rat
