Technical Data

550797
Clone Type
Monoclonal
Host
Rat
Source
Human
Isotype
IgG2a
Clone Number
1D3/TDP-43
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Rt
Accession #
NP_031401
Gene ID
23435
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rat Anti-TAR DNA Binding Protein 43, phosphorylated (Ser409/Ser410) (TARDBP, TDP-43, TDP43)

TDP43 (aka TARDBP) was originally identified as a protein which binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins which bind single stranded RNA. TDP-43 localizes to the nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. Hyperphosphorylated, ubiquitinated, and cleaved in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
Rat Brain nuclear lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to the C-terminal domain of human TDP43; and inclusive of phosphorylated serine residues 409 and 410.
Form
Supplied as a liquid in PBS. No preservative added.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human TDP-43 when phosphorylated at Ser409/Ser410. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Neumann, M., et al., Phosphorylation of S409/410 of TDP-43 is a consistent feature in all sporadic and familial forms of TDP-43 proteinopathies. Acta Neuropathol 117(2):137-149; 2009. 2. Sarroukh R, et al. 2011. Cell Mol Life Sci. 68:1429. 3. Ramakrishnan M, et al. 2009. Biochemistry 48:10405. 4. Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:10323. 5. Diomede L, et al. 2014. Neurobiol Dis. 62:521.
USBio References
No references available
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