Technical Data

552463
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
4H9A5
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-ATG4B
APG4B; AUTL1

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death and aging. Reduced levels of autophagy have been described in some malignant tumors and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant protein fragment corresponding to aa1-221 from human ATG4B, expressed in E. coli.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Purified
Specificity
Recognizes human ATG4B.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Oncotarget. 2016 Oct 11;7(41):66970-66988. |2. Blood. 2014 Jun 5;123(23):3622-34.
USBio References
No references available
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