Technical Data

552490
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
2G6B2
Grade
Purified
Applications
E FC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-BAX
BCL2L4

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero or homodimers and act as anti or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Applications
Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant protein fragment corresponding to human BAX, expressed in E. coli.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Purified
Specificity
Recognizes human BAX

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Medicine (Baltimore). 2019 Jan;98(4):e14130. |2. Cell Cycle. 2017;16(21):2108-2118.
USBio References
No references available
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