Technical Data

552672
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
5C2G9
Grade
Purified
Applications
E FC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD209
CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

Applications
Suitable for use in ELISA, Flow Cytometry and Western Blot . Other applications not tested.
Recommended Dilution
ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant protein fragment corresponding to aa270-404 from human CD209, expressed in E. coli..
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Purfied
Specificity
Recognizes human CD209.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Blood. 2015 Oct 15;126(16):1911-20. |2. PLoS One. 2014 Aug 22;9(8):e105236.
USBio References
No references available
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