Technical Data

553500
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-His-Tag (6xHis)
Applications
Suitable for use in ELISA, Western Blot and Imnohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to HHHHHH of His-tag.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes 6xHis-tag.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lu P et al. (2015) Modeling-Enabled Characterization of Novel NLRX1 Ligands. |PLoS ONE 10(12): e0145420. doi:10.1371/journal.pone.0145420, 2. Chang, How-Yi et al.“Processing Is Required for a Fully Functional Protein P30 in Mycoplasma Pneumoniae Gliding and Cytadherence .”Journal of Bacteriology 193.20 (2011): 5841–5846. PMC. Web. 10 Feb. 2018, 3. Relich RF et al.|Insights into the function of Mycoplasma pneumoniae protein P30 from orthologous gene replacement Microbiology, October 2011 157: 2862-2870, doi: 10.1099/mic.0.052464-0,, 4. Mosnier LO et al. Hyperantithrombotic, noncytoprotective Glu149Ala-activated protein C mutant. Blood. 2009;113(23):5970-5978. doi:10.1182/blood-2008-10-183327., 5. Krylova I et al. Structural Analyses Reveal Phosphatidyl Inositols as Ligands for the NR5 Orphan Receptors SF-1 and LRH-1 Cell , Volume 120 , Issue 3 , 343 - 355
USBio References
No references available
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