Rabbit Anti-NDRG1
Protein NDRG1 (Differentiation-related gene 1 protein) (DRG-1) (N-myc downstream-regulated gene 1 protein) (Nickel-specific induction protein Cap43) (Reducing agents and tunicamycin-responsive protein) (RTP) (Rit42), NDRG1, CAP43 DRG1 RTP
Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53- dependent mitotic spindle checkpoint. Regulates microtubule dynamics andmaintains euploidy.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:5000 Immunohistochemistry (Paraffin): 1:200-1:500. Heat antigen retrieval is required using citrate buffer, pH 6.0. Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa136-394 of human NDRG1. Subcellular Location: Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nucleus, Cell membrane
Form
Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human NDRG1
