Mouse Anti-B7H3
B7-H3: CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, CD276
CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
Applications
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry (paraffin): 2ug/ml Immunocytochemistry: 1ug/ml Immunofluorescence: 10ug/ml Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HEK293 Cell Lysate, HEK293 Cell Slide
Molecular Weight
~57kD (predicted), 100kD (observed)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Extracellular domain of human B7H3.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human B7H3.
