Mouse Anti-LAG3
LAG3: lymphocyte activating 3, LAG-3, CD223
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
Applications
Suitable for use in Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunocytochemistry: 1ug/ml Immunofluorescence: 10ug/ml Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Lymphoma Tissue Lysate, Human Spleen Tissue
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Extracellular domain of human LAG3.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human LAG3.
