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583204 Mouse Anti-LAG3

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P18627
Isotype
IgG1,k
Clone Number
6D1
Grade
Affinity Purified
Applications
IC IF IHC
Crossreactivity
Hu
Accession #
NP_002277
Gene ID
3902
Shipping Temp
Blue Ice
Storage Temp
-20°C
LAG3: lymphocyte activating 3, LAG-3, CD223

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Applications
Suitable for use in Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunocytochemistry: 1ug/ml Immunofluorescence: 10ug/ml Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Lymphoma Tissue Lysate, Human Spleen Tissue
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Extracellular domain of human LAG3.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human LAG3.
References
1. Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.|2. Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.|3. Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.|4. Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.
USBio References
No references available
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