Rabbit Anti-NKX2-8
NKX2-8: NKX2H, NKX2.8, Nkx2-9, NKX-2.8, NKX2G, NKX2H, Homeobox protein NK-2 homolog H
NKX2-8 (NK2 homeobox 8) is a member of a family of transcription factors that are involved in embryonic development and cell fate. It is expressed in the ventral foregut, the developing heart, the epithelial layers of the branchial arches and in the dorsal mesoderm. In conjunction with related protein, NKX2-5, NKX2-8 may play a role in cardiac embryonic development. NKX2-8 is also thought to be involved in lung development and is suspected of being an oncogene in lung cancer that is activated by way of gene amplification at chromosome 14q13.
Applications
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml immunohistochemistry (paraffin): 2.5ug/ml Immunofluorescence: 2.5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Rat Liver Tissue Lysate, Human Liver Tissue Slide
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 19 amino acids, located within aa60-110, near the center of human NKX2-8.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NKX2-8. Species Crossreactivity: mouse and rat
