Technical Data

583247
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_036382
Gene ID
22800
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RRAS2
RRAS2: TC21, TC21, Ras-related protein R-Ras2, Ras-like protein TC21

Activating mutations and overexpression of classical Ras subfamily members (K-RAS, N-RAS and H-RAS) have been widely investigated as key events in the development of human cancers. The RRAS subfamily of Ras-related proteins includes RRAS1, RRAS2 (TC21) and RRAS3 (M-Ras) show overall amino acid identity with the classical Ras subfamily (H-Ras, K-Ras and N-Ras) of 55–60%. RRAS2 is a small GTPbinding protein of the Ras superfamily of GTPases. It might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins. RRAS2 has high oncogenic potential and overexpression/mutations have been reported in several tumor tissues and cell lines.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat Cell Lysate
Molecular Weight
~22kD
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 16 amino acid near the C-terminus of human RRAS2.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human RRAS2. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ehrhardt A, Ehrhardt GR, Guo X, et al. Ras and relatives--job sharing and networking keep an old family together. Exp. Hematol. 2002; 30:1089-106.|2. Lowe DG, Capon DJ, Delwart E, et al. Structure of the human and murine R-Ras genes, novel genes closely related to Ras proto-oncogenes. Cell 1987; 48:137-46|3. Drivas GT, Shih A, Coutavas E, et al. Characterization of four novel Ras-like genes expressed in a human teratocarcinoma cell line. Mol. Cell. Biol. 1990;10:1793-8.|4. Luo H, Hao X, Ge C, et al. TC21 promotes cell motility and metastasis by regulating the expression of E-cadherin and N-cadherin in hepatocellular carcinoma. Int. J. Oncol. 2010; 37:853-9
USBio References
No references available
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