Technical Data

A0001-35
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_057245.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-A4GNT (Alpha-1,4-N-acetylglucosaminyltransferase, Alpha4GnT, MGC149493)
EC=2.4.1.-

This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.

Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:8000 Western Blot: 1-3ug/ml ~40kD band observed in lysates of cell line K562 (calculated MW of 39.5kD according to NP_057245.1) Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-PRTYRDLIKGPEGS, from the C-Terminus of human A4GNT (NP_057245.1).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human A4GNT. Species sequence homology: canine, bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ishizone S, Yamauchi K, Kawa S, Suzuki T, Shimizu F, Harada O, Sugiyama A, Miyagawa S, Fukuda M, Nakayama J, Clinical utility of quantitative RT-PCR targeted to alpha1,4-N-acetylglucosaminyltransferase mRNA for detection of pancreatic cancer. Cancer science 2006 Feb 97 (2): 119-26.
USBio References
No references available
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