A0224
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
FC IF IHC IP WBCrossreactivity
Bo Hu Mk Mo RtGene ID
31, 32Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Acetyl CoA Carboxylase (ACC1)
Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACCa is the predominant isoform in liver, adipocytes and mammary gland, while the 280kD ACCb is the major isoform in skeletal muscle and heart. Phosphorylation by AMPK at Ser79, or by PKA at Ser1200, inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.
Applications
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50 Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. Antigen retrieval with citrate buffer is recommended. Immunoprecipitation: 1:50 Immunofluorescence (IC): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Ser523 of human acetyl CoA carboxylase a1. MW of Antigen: 280kD. Species Sequence Homology: chicken and Drosophila 100%.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of all isoforms of human acetyl CoA carboxylase protein at ~280kD. Species Crossreactivity: bovine, mouse, rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Jianqiang M, et al. PNAS 100:7515-7520, 2003 2. Girad J, et al. The FASEB Journal 8:36-42, 1994 3. Wakil SJ, et al. Annu Rev Biochem 52:537–579, 1983 4. Abu-Elheiga L, et al. Science 291: 2613-2616. 200 5. Ruderman, N.B. et al., Am. J. Physiol. 276, E1–E18, 1999 6. Ha, J. et al., J. Biol. Chem. 269, 22162–22168, 1994 7. Abu-Elheiga, L. et al., Science 291, 2613–2616, 2001 8. Levert, K.L. et al,. J. Biol. Chem. 277, 16347–16350, 2002USBio References
No references available