Technical Data

A0526-31
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Purified
Applications
E FC IC
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-Acetylcholine, Conjugated
Applications
Suitable for use in ELISA, Immunocytochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Crossreactivity Data
Using a conjugate Choline-GA-PC, antibody specificity was performed with an ELISA test by competion experiments with the following compounds.
Compound Crossreactivity ratio (a) Choline-PC   1 Phosphatidylcholine (free) >1:10,000 Choline (free) >1:10,000 Acetylcholine (free) >1:10,000
(a): Choline-PC concentration/unconjugated or conjugated analogs concentration at half displacement.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic choline conjugated to protein carrier (PC).
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH20.
Purity
Purified
Specificity
Recognizes protein conjugated Acetylcholine. See additional crossreactivity data.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Geffard M, Vieillemaringe J, Heinrich-Rock AM & Duris P, “Anti-acetylcholine antibodies and first immunocytochemical application in insect brain”, Neurosci. Lett., vol. 57: 1-6, 1985. 2. Geffard M, Mc Rae-Degueurce A & Souan ML, “Immunocytochemical detection of acetylcholine in the rat central nervous system”, Science, vol. 229: 77-79, 1985. 3. Mc Rae-Degueurce A & Geffard M, “One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain”, Brain Res., vol. 376: 217-219, 1986. 4. Wang-Bennett LT, Souan ML & Glantz RM, “Immunocytochemical studies of the distribution of acetylcholine in the crayfish”, J. Comp. Neurol., vol. 273: 330-343, 1988. 5. Hoogduijn MJ, Cheng A & Genever PG, “Functional nicotinic and muscarinic receptors on mesenchymal stem cells”, Stem Cells Dev., vol. 18: 103-112, 2008. 6. Profita M, Bonanno A, Siena L, Bruno A, Ferraro M, Montalbano AM, Albano GD, Riccobono L, Casarosa P, Pieper MP & Gjomarkaj M, “Smoke, choline acetyltransferase, muscarinic receptors and fibroblast proliferation in chronic obstructive pulmonary disease”, The Journal of Pharmacology and Experimental Therapeutic, vol. 329: 753-63, 2009. 7. Zhang G, Kernan KA, Thomas A, Collins S, Song Y, Li L, Zhu W, Leboeuf RC & Eddy AA, “A novel signalling pathway. Fibroblast nicotinic receptor α1 binds urokinase and promotes renal fibrosis”, The Journal of Biological Chemistry, vol. 284: 29050-64, 2009.
USBio References
No references available
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