Rabbit Anti-Acid Sensitive Ion Channel 3 (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) (Not for Export EU)
Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC), (3) and mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride. The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASIC for Acid Sensing Ion Channels). Three are at least three distinct proteins in ASIC family: ASIC1 (identical with human BNAC2 or BNC2), expressed in brain and dorsal root ganglions (DRG) cells, is activated by pH <7.0.
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide. Optimal dilutions to be determined by the researcher.
Recommended Control Peptide
A0576-06: Acid Sensitive Ion Channel 3, Rat, CT, 21aa (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) Control Peptide.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 21aa sequence near the cytoplasmic C-terminus of rat DRASIC. (KLH coupled).
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity
Recognizes rat Acid Sensitive Ion Channel 3. No significant sequence homology with other ASICs. Species sequence homology: human: 95%; human testis specific DRASIC homolog hTNaCl: 90%.
