Rabbit Anti-Acinus (AAE7, ACN1, AC NON-UTILIZING 1, ACYL-ACTIVATING ENZYME 7, AT3G16910.1, Apoptotic Chromatin condensation inducer in nucleus)
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’ (2).
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml. K562 cell lysate (Cat # K0001-25) can be used as positive control 100ug at 2mg/ml and an approximate 220kD band can be detected. Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Peptide (TRTALHGVKWPQSNPK) corresponding to aa 1065 to 1080 of human AcinusL, 338 to 353 of human AcinusS’, or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is located near the C-terminus of the cleaved active peptide p17.
Form
Supplied as a liquid in PBS, 0.01% sodium azide, 40% glycerol.
Purity
Immunoaffinity chromatography purified IgG
Specificity
Detects Acinus
