Rabbit Anti-ADAMTS4 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 4, ADAM-TS 4, ADAM-TS4, ADAMTS-4, Aggrecanase-1)
ADAMTS4 is a human gene that encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS is a novel family of extracellular proteases found in both mammals and invertebrates. Members of the family may be distinguished from the ADAM (adisintegrin and metalloprotease) family members based on the multiple copies of thrombospondin 1-like repeats they carry. Pratta et al. (2003) concluded that ADAMTS4 is constitutively produced in monolayer chondrocytes, capsular fibroblasts, and cartilage, and that stimulation by interleukin-1 results in aggrecanase activation. Thus, the activator could be a potential target by which to control aggrecanase-mediated degradation in arthritic diseases.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot:1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to a sequence at the C-terminal of |mouse ADAMTS4, different to the related rat sequence by 3 aa.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ADAMTS4. Species Cross-Reactivity: rat.
