A0859-61Z1-HRP
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
HRPIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_620594Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rabbit Anti-ADAMTS13, ID (von Willebrand Factor-cleaving Protease, vWF-cleaving Protease, vWF-CP, A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13, ADAMTS-13, ADAM-TS 13, ADAM-TS13, UNQ6102/PRO20085, C9orf8) (Azide free) (HRP)
ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.
Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 829-858 in the central region of human ADAMTS13.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ADAMTS13.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Zheng X., Chung D., Takayama T.K.J. Biol. Chem. 276:41059-41063(2001). 2. Levy G.G., Nichols W.C.Nature 413:488-494(2001). 3. Cal S., Obaya A.J., Llamazares M., Garabaya C.Gene 283:49-62(2002). 4. Zheng X., Nishio K., Majerus E.M.J. Biol. Chem. 278:30136-30141(2003). 5. Anderson P.J., Kokame K., Sadler J.E.J. Biol. Chem. 281:850-857(2006). 6. Pimanda J.E., Maekawa A., Wind T.Blood 103:627-629(2004). 7. Plaimauer B., Fuhrmann J., Mohr G.Blood 107:118-125(2006).USBio References
No references available