Technical Data

A0859-61Z1
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_620594; Q76LX8
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ADAMTS13, ID (von Willebrand Factor-cleaving Protease, vWF-cleaving Protease, vWF-CP, A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13, ADAMTS-13, ADAM-TS 13, ADAM-TS13, UNQ6102/PRO20085, C9orf8)

ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 829-858 in the central region of human ADAMTS13.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ADAMTS13.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Zheng X., Chung D., Takayama T.K.J. Biol. Chem. 276:41059-41063(2001). 2. Levy G.G., Nichols W.C.Nature 413:488-494(2001). 3. Cal S., Obaya A.J., Llamazares M., Garabaya C.Gene 283:49-62(2002). 4. Zheng X., Nishio K., Majerus E.M.J. Biol. Chem. 278:30136-30141(2003). 5. Anderson P.J., Kokame K., Sadler J.E.J. Biol. Chem. 281:850-857(2006). 6. Pimanda J.E., Maekawa A., Wind T.Blood 103:627-629(2004). 7. Plaimauer B., Fuhrmann J., Mohr G.Blood 107:118-125(2006).
USBio References
No references available
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