Mouse Anti-Adeno-Associated Virus (AAV) VP1, VP2, VP3
Useful for immunolocalization studies of capsid formation in combination with monoclonal A0868-35 and rabbit polyclonal antibody to AAV capsid proteins (Cat.# A0868-36N). Clone B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wo- bus et al., see below) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Dot Blot, Immunoprecipitation and Affinity chromatography. Other applications have not been tested.
Recommended Dilutions
Immunoprecipitation: Mainly free VP proteins. Western Blot: 1:250-1:500 (0.1-0.2ug/ml) Dot Blot: 1:500 (0.1ug/ml; denaturing conditions) Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Adeno-associated virus 2 capsid proteins and virus particles.
Form
Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes free Adeno-Associated Virus 2 VP1, VP2, VP3 found in human. Cross-reactive with AAV-1, AAV-3, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAVDJ and AAVrh-10.
