Rabbit Anti-Adiponectin Receptor 1 (AdipoR1, CGI-45)
The adiponectin receptors, ADIPOR1 and ADIPOR2, serve as receptors for globular and full-length adiponectin and mediate increased AMPK and PPAR-alpha (PPARA) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin. ADIPOR1 and ADIPOR2 are highly related structurally, and mouse Adipor1 and Adipor2 share 66.7% identity. ADIPOR1 and ADIPOR2 are 7-transmembrane domain proteins, but they are structurally, topologically, and functionally distinct from G proteincoupled receptors (GPCRs). The mouse, human, and rat Adipor1 protein (ADIPOR1) contains 375 amino acids (with a predicted MW of 42.4 kD). Human and mouse ADIPOR1 have 96.8% homology. ADIPOR1 has high-affinity receptor for globular adiponectin (gACRP30) but low-affinity receptor for full-length adiponectin. ADIPOR1 is widely expressed. It is highly expressed in skeletal muscle. It is expressed at intermediate levels in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. It is weakly expressed in colon, thymus and small intestine.
Applications
Suitable for use in Western Blot, Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1-10ug/ml using ECL ELISA: 0.5-1ug/ml. Use 1ug/ml of control peptide to coat ELISA plates. Immunofluorescence: 2-20ug/ml Optimal dilutions to be determined by the researcher.
Control Peptide
A0886-75E: Adiponectin Receptor 1, Mouse, Control Peptide
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide corresponding to 18aa from the C-terminus, extracellular domain of mouse ADIPOR1, conjugated to KLH. Species Sequence Homology: Green puffer, porcine, duck, chicken, rat, bovine, 100%; human, chimp, frog, and zebra fish, 94%.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Adiponectin Receptor 1. No significant sequence homology is seen with ADIPOR2.
