Technical Data

A0890A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Adipsin (Factor D) (Not for Export EU)

Adipsin is serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Adipsin, also called ADN or complement factor D or C3 convertase activator or properdin factor D (precursors: mouse 259-aa; rat 263 aa, human 253 aa, mature protein 26-253, ~22kD) cleaves factor B when the latter is complexed with factor C3B, activating the C3BB complex, which then becomes C3 convertase of the alternative pathway. Adipocyte is the major protein secreted by the adipocytes. Unlike rodents, adipsin is also expressed in monocytes/macrphages. Most adipsin is secreted in blood (50ug/ml in normal lean mice and 50-100 fold less in fat from db/db or ob/ob or MSG (monosodium glutamate-treated mice). Its expression is induced upon differentiation of preadipocytes.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:5000. Adipsin is ~22kD. ELISA: 1:10-50,000; 10-100ng of control peptide/well). Optimal dilutions to be determined by the researcher.
Recommended Control Peptide
A0890-01: Adipsin Human Control Peptide
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 18aa near the N-Terminus of human adipsin, conjugated to KLH. Species Sequence Homology: chimp; 95%, bovine; 80%, porcine; 78%, rat; 0%, mouse; 63%.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Purity
Serum
Specificity
Recognizes human Adipsin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. White RT et al (1992) JBC 267, 9210; 2. Niemann MA et al (1984) Biochem. 23, 2482; 3. Johnson DM et al (1980) Bicohem. J. 187, 863; 4. Volanakis Je et al (1980) PNAS 77, 1116; 5. Zhu L et al (1994) J. Clin. Invest. 94, 1163; 6. Baker BC et al (1991) 279, 775; 7. Min HY et al (196) Nucl Acid Res. 14, 8879;
USBio References
No references available
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