Technical Data

A0906-64F
Clone Type
Polyclonal
Host
Goat
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E IF
Crossreactivity
Ca Hu Mo Rt
Accession #
NP_000672.2
Gene ID
150
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-ADRA2A (Alpha-2A Adrenergic Receptor, Alpha-2 Adrenergic Receptor Subtype C10, Alpha-2A Adrenoreceptor, Alpha-2A Adrenoceptor, Alpha-2AAR, ADRA2R, ADRAR)

Alpha-2-adrenergic receptor (ADRA2), also known as platelet type adrenoceptor alpha-2A, is a member of G protein-coupled receptor superfamily. This gene, which can identify a DraI RFLP of the ADRAR gene, mapped to 10q23-q25, is found in the distal region of mouse chromosome 19 and abundantly expressed in giant cell. ADRA2 acts a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. It has 3 highly homologous subtypes: ADRA2A; ADRA2B and ADRA2C. Studies in mouse suggested that both the ADRA2A and ADRA2C subtypes are required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. ADRA2A receptors inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.

Applications
Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-TERRPNGLGPERS, from mouse ADRA2A, at the internal region of the protein (NP_000672.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ADRA2A. Species sequence homology: Canine, human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Tan M, Walwyn WM, Evans CJ, Xie CW. p38 MAPK and beta-arrestin 2 mediate functional interactions between endogenous micro-opioid and alpha2A-adrenergic receptors in neurons. J Biol Chem. 2009 Mar 6;284(10):6270-81. Epub 2009 Jan 6.
USBio References
No references available
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