Rabbit Anti-Akt (PKB, RAC, Related to PKA and PKC)
Akt (PKB, or RAC (Related to PKA and PKC), is the cellular homologue of the viral oncoprotein v-Akt. Akt is a serine / threonine kinase that consists of an N-terminal PH domain, a kinase domain and a C-terminal domian similar to that of the PKC family. Akt alpha contains 480 amino acids, and can be activated by receptor tyrosine kinases through the PI 3-kinase pathway. Akt has been shown to suppress apoptosis. Recent study suggests that Akt may also be part of the NF-kB activation pathway.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-expressed mouse Akt alpha (aa 375-479)
Form
Supplied as a lyophilized powder from 0.09% sodium azide. Reconstitute with 200ul sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse and human Akt/PKB.
